Cloned (Comment) | Organism |
---|---|
gene BDH1, construction of plasmid B1ZF-loxP-KanMX-loxPPGK1p-BDH1-PGK1t-B1ZR in Escherichia coli strain DH5alpha, and recombinant overexpression in Saccharomyces uvarum strain WY1 resulting in strain WY1-1, overexpression of gene BDH2 from Saccharomyces uvarum (WY1-2), method overview. BDH1 and BDH2 overexpression in one strain, named WY1-12, is constructed on the basis of WY1-2, that is, the strain WY1-2 is transformed into plasmid Yep-KPB1 after excising KanMX, real-time PCR expression analysis | Saccharomyces uvarum |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of a an BDH1 deletion strain, WY1DELTA1, which shows 1.37fold increased diacetyl production | Saccharomyces uvarum |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | Saccharomyces uvarum | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | Saccharomyces uvarum WY1 | - |
(R,R)-butane-2,3-diol + NAD+ | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces uvarum | - |
- |
- |
Saccharomyces uvarum WY1 | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(R)-acetoin + NADH + H+ | - |
Saccharomyces uvarum | (R,R)-butane-2,3-diol + NAD+ | - |
r | |
(R)-acetoin + NADH + H+ | - |
Saccharomyces uvarum WY1 | (R,R)-butane-2,3-diol + NAD+ | - |
r |
Synonyms | Comment | Organism |
---|---|---|
2,3-butanediol dehydrogenase | - |
Saccharomyces uvarum |
BDH1 | - |
Saccharomyces uvarum |
Bdh1p | - |
Saccharomyces uvarum |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Saccharomyces uvarum | |
NADH | - |
Saccharomyces uvarum |
Organism | Comment | Expression |
---|---|---|
Saccharomyces uvarum | BDH2 is a gene adjacent to BDH1, and these genes are regulated reciprocally | additional information |
General Information | Comment | Organism |
---|---|---|
metabolism | BDH2 is a gene adjacent to BDH1, and these genes are regulated reciprocally. BDH2 is only responsible for converting diacetyl into acetoin, but not for the metabolic pathway of acetoin to 2,3-butanediol in Saccharomyces uvarum | Saccharomyces uvarum |
physiological function | the enzyme is involved in formation of diacetyl in wine | Saccharomyces uvarum |